Cell Bio Lab 1_Protein Quantification

Describe how Coomassie brillian

t

blue G

250 dye can be used to detect proteins in

solution

Determine how a set of known protein sample concentrations can be utilized to

determine the concentration of an unknown sample through the use of

a Coomassie

colori

metric assay

Identify the unknown sample dilution(s) that allow

s

you to most confidently determine

the protein concentration

of your unknown

Calculate the unknown sample’s concentration taking into consideration dilution factors

,

where needed

Identify pot

ential sources of variation

in the experimental procedure and establish

techniques to help minimize experimental error

Suggest when to use serial vs parallel dilutions and why each can be beneficial

depending

on the

particular experimental conditions

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