Describe how Coomassie brillian
250 dye can be used to detect proteins in
Determine how a set of known protein sample concentrations can be utilized to
determine the concentration of an unknown sample through the use of
Identify the unknown sample dilution(s) that allow
you to most confidently determine
the protein concentration
of your unknown
Calculate the unknown sample’s concentration taking into consideration dilution factors
ential sources of variation
in the experimental procedure and establish
techniques to help minimize experimental error
Suggest when to use serial vs parallel dilutions and why each can be beneficial
particular experimental conditions
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